Attenuated West Nile Virus Mutant NS1 130-132QQA/175A/207A Exhibits Virus-Induced Ultrastructural Changes and Accumulation of Protein in the Endoplasmic Reticulum-Reference-Cited by-同舟云学术

Attenuated West Nile Virus Mutant NS1 130-132QQA/175A/207A Exhibits Virus-Induced Ultrastructural Changes and Accumulation of Protein in the Endoplasmic Reticulum

Published:2015-01-15 Issue:2 Volume:89 Page:1474-1478
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Whiteman Melissa C.¹,Popov Vsevolod²³,Sherman Michael B.⁴⁵,Wen Julie²,Barrett Alan D. T.¹²³⁶

Affiliation:

1. Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas, USA

2. Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA

3. Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA

4. Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA

5. Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, Texas, USA

6. Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA

Abstract

ABSTRACT We have previously shown that ablation of the three N-linked glycosylation sites in the West Nile virus NS1 protein completely attenuates mouse neuroinvasiveness (≥1,000,000 PFU). Here, we compared the replication of the NS1 130-132QQA/175A/207A mutant to that of the parental NY99 strain in monkey kidney Vero cells. The results suggest that the mechanism of attenuation is a lack of NS1 glycosylation, which blocks efficient replication, maturation, and NS1 secretion from the endoplasmic reticulum and results in changes to the virus-induced ultrastructure.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.02215-14

Reference22 articles.

1. Development and characterization of non-glycosylated E and NS1 mutant viruses as a potential candidate vaccine for West Nile virus

2. Multiple amino acid changes at the first glycosylation motif in NS1 protein of West Nile virus are necessary for complete attenuation for mouse neuroinvasiveness

3. Determination of the intramolecular disulfide bond arrangement and biochemical identification of the glycosylation sites of the nonstructural protein NS1 of Murray Valley encephalitis virus;Blitvich BJ;J Gen Virol,2001

4. Dengue Virus Type 1 Nonstructural Glycoprotein NS1 Is Secreted from Mammalian Cells as a Soluble Hexamer in a Glycosylation-Dependent Fashion

5. Maturation of Japanese encephalitis virus glycoproteins produced by infected mammalian and mosquito cells

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