Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella-Reference-Cited by-同舟云学术

Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella

Published:2004-05 Issue:5 Volume:42 Page:2261-2263
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bogdanovich T.¹,Skurnik M.²,Lübeck P. S.³,Ahrens P.³,Hoorfar J.³

Affiliation:

1. University of Turku, Turku

2. Haartman Institute, University of Helsinki, Helsinki, Finland

3. Danish Institute for Food and Veterinary Research, Copenhagen, Denmark

Abstract

ABSTRACT A real-time, genus-specific 5′ nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains ( n = 23) and did not detect non- Brucella strains ( n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.5.2261-2263.2004

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