Use of the omp50 Gene for Identification of Campylobacter Species by PCR-Reference-Cited by-同舟云学术

Use of the omp50 Gene for Identification of Campylobacter Species by PCR

Published:2004-05 Issue:5 Volume:42 Page:2301-2305
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Dedieu Luc¹,Pagès Jean-Marie¹,Bolla Jean-Michel¹

Affiliation:

1. EA 2197 and IFR 48, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 5, France

Abstract

ABSTRACT We studied the prevalence of the omp50 gene and the Omp50 protein in Campylobacter strains. Immunodetection assays and DNA-DNA hybridizations showed that most C. coli strains tested were negative and most C. jejuni and C. lari strains tested were positive. A PCR assay was developed, using the omp50 gene as a species-specific target. We propose a combination of a hippurate test and an omp50 assay to perform identification of Campylobacter species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.5.2301-2305.2004

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