Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes-Reference-Cited by-同舟云学术

Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

Published:2004-05 Issue:5 Volume:42 Page:2294-2297
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Garcia Lineth¹²,Kindt Ann¹,Bermudez Hernan²,Llanos-Cuentas Alejandro³,De Doncker Simonne¹,Arevalo Jorge³,Quispe Tintaya Kelly Wilber¹³,Dujardin Jean-Claude¹

Affiliation:

1. “Prince Leopold” Institute of Tropical Medicine, Molecular Parasitology, Antwerp, Belgium

2. Centro Universitario de Medicina Tropical, Cochabamba, Bolivia

3. Instituto de Medicina Tropical “Alexander von Humboldt,” Lima, Peru

Abstract

ABSTRACT PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.5.2294-2297.2004

Reference12 articles.

1. Arora, S. K., G. S. Kapoor, and S. Sehgal. 1998. Heterogeneity in heat shock protein genes in Leishmania isolates. Immunol. Cell Biol.76:186-189.

2. Bañuls A. L. 1998. Apport de la génétique évolutive à la taxonomie et à l'épidémiologie du genre Leishmania. Ph.D. thesis. University of Montpellier Montpellier France.

3. Bastrenta, B., R. Buitrago, F. Vargas, F. Le Pont, M. Torrez, M. Flores, N. Mita, and S. F. Breniere. 2002. First evidence of transmission of Leishmania (Viannia) lainsoni in a Sub Andean region of Bolivia. Acta Trop.83:249-253.

4. Bastrenta, B., N. Mita, R. Buitrago, F. Vargas, M. Flores, M. Machane, N. Yacsik, M. Torrez, F. Le Pont, and S. F. Brenière. 2003. Human mixed infections of Leishmania spp. and Trypanosoma cruzi in a Sub Andean Bolivian area: identification by polymerase chain reaction/hybridization and isoenzyme. Mem. Inst. Oswaldo Cruz98:255-264.

5. Cupolillo, E., G. Grimaldi, Jr., H. Momen, and S. M. Beverley. 1995. Intergenic region typing (IRT), a rapid molecular approach to the characterization and evolution of Leishmania.Mol. Biochem. Parasitol.73:145-155.

Cited by 166 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Unveiling the Enigmatic nature of six neglected Amazonian Leishmania (Viannia) species using the hamster model: Virulence, Histopathology and prospection of LRV1;PLOS Neglected Tropical Diseases;2024-08-09

2. Detection of Internal Transcribed Spacer 1 and hsp70 Genetic Markers Using Restriction Fragment Length Polymorphisms and Sequencing in Identification of Leishmania Species Causing Tegumentary Leishmaniasis in Brazil;The American Journal of Tropical Medicine and Hygiene;2024-01-03

3. Leishmania (Sauroleishmania) tarentolae versus pathogenic species: comparative evaluation of protease activity, glycoconjugates, resistance to complement and metabolome composition;Memórias do Instituto Oswaldo Cruz;2024

4. Monitoring Cutaneous Leishmaniasis Lesions in Mice Undergoing Topical Miltefosine Treatment;Scientia Pharmaceutica;2023-11-09

5. A fine mapping of single nucleotide variants and haplotype analysis of IL13 gene in patients with Leishmania guyanensis-cutaneous leishmaniasis and plasma cytokines IL-4, IL-5, and IL-13;Frontiers in Immunology;2023-10-16