Transposition of Tn917 in Bacillus megaterium

Author:

Bohall N A,Vary P S

Abstract

Transposon Tn917, carried on plasmid pTV1, was introduced into Bacillus megaterium and transposed efficiently and apparently randomly. Insertional mutations included at least eight different auxotrophic loci, two carbon source loci, and sporulation loci. One trp::Tn917 mutation was further verified as an insertion by both reversion and transduction.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference18 articles.

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