Affiliation:
1. Institut für Hygiene und Mikrobiologie, Universität Würzburg
2. Universitätsklinik für Frauenheilkunde
3. Dermatologische Universitätsklinik, 97080 Würzburg, Germany
Abstract
ABSTRACT
In this work we analyzed the roles of meningococcal lipooligosaccharide (LOS) and capsule expression in the interaction of
Neisseria meningitidis
with human dendritic cells (DC). Infection of DC with serogroup B wild-type meningococci induced a strong burst of the proinflammatory cytokines and chemokines tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-8. In contrast, a serogroup B mutant strain lacking LOS expression barely led to cytokine induction, demonstrating that meningococcal LOS is the main mediator of the proinflammatory response in human DC. Sialylation of meningococcal LOS did not influence cytokine secretion by DC. However, we found the phagocytosis of
N. meningitidis
by human DC to be inhibited by LOS sialylation. In addition, the expression of the meningococcal serogroup A, B, and C capsules dramatically reduced DC adherence
of N. meningitidis
and phagocytosis to some extent. Hence, LOS sialylation and capsule expression are independent mechanisms protecting
N. meningitidis
from the phagocytic activity of human DC.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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