Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coli-Reference-Cited by-同舟云学术

Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coli

Published:2004-02 Issue:2 Volume:70 Page:1238-1241
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Kim Pil¹,Laivenieks Maris¹,Vieille Claire¹,Zeikus J. Gregory¹

Affiliation:

1. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824

Abstract

ABSTRACT Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc :: kan , PEPCK overexpression increased succinate production 6.5-fold.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.2.1238-1241.2004

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