Arenavirus Concentration by Molecular Filtration

Author:

Gangemi J. D.1,Connell E. V.1,Mahlandt B. G.1,Eddy G. A.1

Affiliation:

1. United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701

Abstract

Liter volumes of a human arenavirus pathogen (Machupo) and a nonpathogen (Tacaribe) were concentrated 30 to 100 times in less than 90 min without significant loss of particle infectivity.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference11 articles.

1. Albertson P. A. 1967. Two-phase separation of vi ruses p. 303-321. In K. Maramorosch and H. Koprowski (ed.) Methods in virology vol. 2. Academic Press Inc. New York.

2. Lymphocytic choriomeningitis virus. I. Concentrations and purification of the infectious virus;Gschwender H. H.;J. Virol.,1975

3. Ultrafiltration as a method for concentrating Rift Valley fever virus grown in tissue culture;Klein F.;Appl. Microbiol.,1971

4. Filtration methods for recovering enteroviruses from foods;Kostenbader K. D.;Appl. Microbiol.,1973

5. Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation;McSharry J.;Virology,1970

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1. References;Perspectives in Medical Virology;1986

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3. Measles virus: Conditions for the propagation and purification of infectious virus in high yield;Journal of Virological Methods;1984-02

4. Large scale isolation of human erythrocyte membranes by high volume molecular filtration;Journal of Biochemical and Biophysical Methods;1981-01

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