Multiplex PCR for Simultaneous Detection of Macrolide and Tetracycline Resistance Determinants in Streptococci

Author:

Malhotra-Kumar Surbhi1,Lammens Christine1,Piessens Jasper1,Goossens Herman1

Affiliation:

1. Belgian Reference Center for Group A Streptococcus, University of Antwerp, Antwerp, Belgium

Abstract

ABSTRACT Resistance to macrolides and tetracyclines is increasing among streptococci and co-occurs as their resistance determinants are carried on the same mobile element. We developed a multiplex PCR to facilitate simultaneous and specific detection of resistance determinants for both macrolides [ erm (A), erm (B), and mef (A/E)] and tetracyclines [ tet (M), tet (O), tet (K), and tet (L)] in streptococci.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

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