On-Line Detection of Substrate Exhaustion by Using NAD(P)H Fluorescence

Author:

Kwong Simon C. W.1,Randers Lisa1,Rao Govind1

Affiliation:

1. Chemical and Biochemical Engineering Program and Medical Biotechnology Center of the Maryland Biotechnology Institute, University of Maryland, Baltimore, Maryland 21228

Abstract

In this study we examined the utility of NAD(P)H fluorescence for monitoring aerobic fermentations of the threonine auxotroph Corynebacterium glutamicum ATCC 14296. Instead of attempting complicated mathematical corrections for inner-filter effects, we found that it is possible to use the information contained in the on-line NAD(P)H fluorescence signal to assess culture metabolic activities during fermentation. The first derivative of the filtered fluorescence signal, which approximates the turnover rate of the NAD(P)H pool, can be used to precisely identify the temporal points of threonine and glucose exhaustion.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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