Affiliation:
1. Department of Genetics, University of Washington, Seattle 98195.
Abstract
The replication of putative replication origins (ARS elements) was examined for 200 kilobases of chromosome III of Saccharomyces cerevisiae. By using synchronous cultures and transfers from dense to light isotope medium, the temporal pattern of mitotic DNA replication of eight fragments that contain ARSs was determined. ARS elements near the telomeres replicated late in S phase, while internal ARS elements replicated in the first half of S phase. The results suggest that some ARS elements in the chromosome may be inactive as replication origins. The actively expressed mating type locus, MAT, replicated early in S phase, while the silent cassettes, HML and HMR, replicated late. Unexpectedly, chromosome III sequences were found to replicate late in G1 at the arrest induced by the temperature-sensitive cdc7 allele.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Reference33 articles.
1. Fine-structure analysis of the DNA sequence requirements for autonomous replication of Saccharomyces cervisiae plasmids;Bouten A. H.;Mol. Cell. Biol.,1986
2. A yeast silencer contains sequences that can promote autonomous plasmid replication and transcriptional activation;Brand A. H.;Cell,1987
3. Time sequence of DNA replication;Braun R.;Physarum. Biochim. Biophys. Acta,1969
4. The localization of replication origins on ARS plasmids in S. cerevisiae;Brewer B. J.;Cell,1987
5. Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae;Buchman A. R.;Mol. Cell. Biol.,1988
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