Modified Map Positions for lac and the pro Markers in Escherichia coli K-12

Author:

Broda Paul1

Affiliation:

1. MRC Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR

Abstract

Interrupted mating experiments were performed with Hfr strains H and C and three leu lac purE recipient strains derived from a common parent and carrying, respectively, the proA , proB , and proC mutations. It was concluded that if leu is placed at 1.5 min and purE at 12 min from thr , the origin on the Taylor-Trotter map, lac is at about 7.5 min and the pro genes are at about 6.0, 6.6, and 8.4 min, respectively. Both conjugational and transductional data suggest that the strain carrying the proB mutation also carries a second mutation close to the proA site which independently confers a Pro phenotype. The times before the onset of transfer of chromosomal deoxyribonucleic acid by both Hfr strains B4 and B8 were approximately 3 min.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference23 articles.

1. Pedigrees of some mutant strains of Escherichia coli K-12;Bachmann B. J.;Bacteriol. Rev.,1972

2. Transposition derivatives of an Hfr strain of Escherichia coli K12;Berg C. M.;Genetics,1967

3. The formation of Hfr strains in Escherichia coli K12;Broda P.;Genet. Res. Camb.,1967

4. Gross map distances and Hfr transfer times in Escherichia coli K-12;Broda P.;J. Bacteriol.,1974

5. Mutants des voies de biosynthese et de degradation de la proline chez E. coli K12;Condamine H.;Ann. Inst. Pasteur,1971

Cited by 13 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3