Affiliation:
1. Faculty of Pharmaceutical Sciences, Kyushu University, Katakasu, Higashi-ku, Fukuoka 812, Japan
Abstract
Two ultraviolet light (UV)-sensitive mutants have been isolated from
Escherichia coli
K-12. These mutants, designated RuvA
−
and RuvB
−
, were controlled by a gene located close to the
his
gene on the chromosome map. They were sensitive to UV (10- to 20-fold increase) and slightly sensitive to gamma rays (3-fold increase). Host cell reactivation, UV reactivation and genetic recombination were normal in these mutants. Irradiation of the mutants with UV resulted in the production of single-strand breaks in deoxyribonucleic acid, which was repaired upon incubation in a growth medium. After UV irradiation, these mutants resumed deoxyribonucleic acid synthesis at a normal rate, as did the parent wild-type bacteria, and formed nonseptate, multinucleate filaments. From these results we concluded that the mutants have some defect in cell division after low doses of UV irradiation, similar to the
lon
−
or
fil
+
mutant of
E. coli
. The
ruv
locus was divided further into
ruvA
and
ruvB
with respect to nalidixic acid sensitivity and the effect of minimal agar or pantoyl lactone on survival of the UV-irradiated cell. The
ruvB
−
mutant was more sensitive to nalidixic acid than were
ruvA
−
and the parent strain. There was a great increase in the surviving fraction of the UV-irradiated
ruvB
−
mutant when it was plated on minimal agar or L agar containing pantoyl lactone. No such increase in survival was observed in the
ruvA
−
mutant.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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