Purification of Single- and Double-Length M13 Virions by Polyacrylamide Gel Electrophoresis

Author:

Beaudoin Jacques12,Henry Timothy J.12,Pratt David12

Affiliation:

1. Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

2. Department of Biophysics and Microbiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213

Abstract

Complete separation of single- and double-length M13 virions from each other and from still-longer phage particles was achieved by electrophoresis of phage stocks through 2.5% polyacrylamide gels.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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