Vaccinia Virus Replication in Enucleate BSC-1 Cells: Particle Production and Synthesis of Viral DNA and Proteins

Author:

Pennington T. H.1,Follett E. A. C.1

Affiliation:

1. Department of Virology and M.R.C. Virology Unit, Institute of Virology, University of Glasgow, Glasgow, Scotland

Abstract

The growth of vaccinia virus in monolayers of BSC-1 cells enucleated by centrifugation in the presence of cytochalasin B has been studied. No evidence for the production of infectious virus in these cells was obtained, and the production of virus particles was reduced to 8.3% compared with the yield from cytochalasin-treated, uncentrifuged cells. Virus DNA and early and late polypeptides were synthesized with normal timing in enucleate cells, but in reduced amounts; cleavage of structural polypeptide precursors P4a and Px also occurred in enucleate cells. Factories containing immature virus particles were demonstrated in enucleate cells by electron microscopy; these factories were reduced in number and size compared with those found in cytochalasin-treated, uncentrifuged cells.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference13 articles.

1. Effects of cytochalasin on mammalian cells;Carter S. B.;Nature (London),1967

2. Analyses of "C-labeled protein by disc electrophoresis;Fairbanks G.;Biochem. Biophys. Res. Commun.,1965

3. Virus replication in enucleate cells: vesicular stomatitis virus and influenza virus;Follett E. A. C.;J. Virol.,1974

4. The purification of four strains of poxvirus;Joklik W. K.;Virology,1962

5. The replication and coating of vaccinia DNA;Joklik W. K.;J. Mol. Biol.,1964

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