Pork Contaminated with
Salmonella
enterica
Serovar 4,[5],12:i:−, an Emerging Health Risk for Humans
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Published:2010-07-15
Issue:14
Volume:76
Page:4601-4610
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ISSN:0099-2240
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Container-title:Applied and Environmental Microbiology
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language:en
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Short-container-title:Appl Environ Microbiol
Author:
Hauser Elisabeth12, Tietze Erhard3, Helmuth Reiner1, Junker Ernst1, Blank Kathrin3, Prager Rita3, Rabsch Wolfgang3, Appel Bernd4, Fruth Angelika3, Malorny Burkhard1
Affiliation:
1. Federal Institute for Risk Assessment, National Reference Laboratory for Salmonella, Diedersdorfer Weg 1, D-12277 Berlin, Germany 2. Free University Berlin, Department of Biology, Chemistry and Pharmacy, Takustrasse 3, 14195 Berlin, Germany 3. Robert Koch Institute, Wernigerode Branch, Division Bacterial Infections, National Reference Centre for Salmonella and Other Enterics, Burgstrasse 37, 38855 Wernigerode, Germany 4. Federal Institute for Risk Assessment, Department of Biological Safety, Diedersdorfer Weg 1, D-12277 Berlin, Germany
Abstract
ABSTRACT
Salmonella enterica
subsp.
enterica
serovar 4,[5],12:i:− is a monophasic variant of
S
.
enterica
serovar Typhimurium (antigenic formula 4,[5],12:i:1,2). Worldwide, especially in several European countries and the United States, it has been reported among the 10 most frequently isolated serovars in pigs and humans. In the study reported here, 148 strains of the monophasic serovar isolated from pigs, pork, and humans in 2006 and 2007 in Germany were characterized by various phenotypic and genotypic methods. This characterization was done in order to investigate their clonality, the prevalence of identical subtypes in pigs, pork, and humans, and the genetic relatedness to other
S. enterica
serovar Typhimurium subtypes in respect to the pathogenic and resistance gene repertoire. Two major clonal lineages of the monophasic serovar were detected which can be differentiated by their phage types and pulsed-field gel electrophoresis (PFGE) profiles. Seventy percent of the strains tested belonged to definite phage type DT193, and those strains were mainly assigned to PFGE cluster B. Nineteen percent of the strains were typed to phage type DT120 and of these 86% belonged to PFGE cluster A. Sixty-five percent of the isolates of both lineages carried core multiresistance to ampicillin, streptomycin, tetracycline, and sulfamethoxazole encoded by the genes
bla
TEM1-like
,
strA
-
str
B
,
tet
(B), and
sul2.
No correlation to the source of isolation was observed in either lineage. Microarray analysis of 61
S. enterica
serovar 4,[5],12:i:− and 20
S. enterica
serovar Typhimurium isolates tested determining the presence or absence of 102 representative pathogenicity genes in
Salmonella
revealed no differences except minor variations in single strains within and between the serovars, e.g., by presence of the virulence plasmid in four strains. Overall the study indicates that in Germany
S. enterica
serovar 4,[5],12:i:− strains isolated from pig, pork, and human are highly related, showing their transmission along the food chain. Since the pathogenicity gene repertoire is highly similar to that of
S. enterica
serovar Typhimurium, it is essential that interventions are introduced at the farm level in order to limit human infection.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference27 articles.
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Salmonella enterica
Serovar Typhimurium and Monophasic
Salmonella
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