Lack of Regulation of the Modification-Dependent Restriction Enzyme McrBC in Escherichia coli-Reference-Cited by-同舟云学术

Lack of Regulation of the Modification-Dependent Restriction Enzyme McrBC in Escherichia coli

Published:2002-03-15 Issue:6 Volume:184 Page:1794-1795
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Murphy Mark¹,Schmid Nuoffer Stefanie¹,Bickle Thomas A.¹

Affiliation:

1. Division of Molecular Microbiology, Biozentrum of the University of Basel, CH-4056 Basel, Switzerland

Abstract

ABSTRACT Restriction alleviation (RA) by the type I restriction enzyme Eco KI is caused by treatments that damage DNA. RA is due to proteolysis of the Eco KI HsdR subunit by the ClpXP ATP-dependent protease. Here we show that the modification-dependent enzyme McrBC is not subject to RA, although it is moderately sensitive to ClpAP.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.184.6.1794-1795.2002

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