Author:
Nakamura T,Yamazaki N,Taniguchi H,Fujimura S
Abstract
Staphylococci from samples of human saliva were isolated on staphylococcal-selective agar plates. These strains were tested for the inhibition of the growth of Staphylococcus aureus FDA209P. The frequency of inhibitory strains among all of the staphylococcal isolates was 5.2%. Strain IYS2, which formed the biggest inhibitory zone against the growth of the indicator strain, was used as the producer of bacteriocin. IYS2 was identified to be S. aureus, based on its biological properties. The bacteriocin was purified by sequential procedures, including ammonium sulfate precipitation, fractionation with ethanol, ion-exchange chromatography, and gel filtration. Its molecular weight was determined to be 5,000. The isoelectric point was 10.0. In amino acid composition, lysine, histidine, aspartic acid, valine, and phenylalanine were predominant. The bacteriocin was heat stable but inactivated by proteases or peptidase. The bacteriocin had a bactericidal effect on susceptible cells. An analysis of the inhibitory spectrum among typical oral indigenous bacteria showed that Streptococcus salivarius, Propionibacterium acnes, Corynebacterium parvulum, and Actinomyces israelii were susceptible to the bacteriocin. Streptococcus mutans, Streptococcus sanguis, Streptococcus mitis, Actinomyces viscosus, Actinomyces naeslundii, Fusobacterium nucleatum, Bacterionema matruchotii, and Bacteroides melaninogenicus were resistant. The majority of S. aureus tested were susceptible, and all Staphylococcus epidermidis strains tested were resistant.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference33 articles.
1. Bacterial interference among strains of Staphylococcus aureus in man;Aly R.;J. Infect. Dis.,1974
2. Estimation of molecular weights of protein by Sephadex gel filtration;Andrews P.;Biochem. J.,1964
3. Baird-Parker A. C. Genus H Staphylococcus Rosenbach 1884 18 nom. cons. Opin. 17 Jud. Comm. 1958 153 p. 483-489. In R. E. Buchanan and N. E. Gibbons (ed.) Bergey's manual of determinative bacteriology 8th ed. The Williams & Wilkins Co. Baltimore.
4. Kinetic studies on the interaction of bacteriophage type 71 staphylococcal bacteriocin with susceptible bacteria;Dajani A. S.;J. Bacteriol.,1973
5. Faddin J. F. M. 1980. Gram-positive bacteria p. 345-370. In Biochemical tests for identification of medical bacteria 2nd ed. Williams & Wilkins Co. Baltimore.
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