Fluorescent Hybridization of Mycobacterium leprae in Skin Samples Collected in Burkina Faso-Reference-Cited by-同舟云学术

Fluorescent Hybridization of Mycobacterium leprae in Skin Samples Collected in Burkina Faso

Published:2020-04-23 Issue:5 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Millogo Anselme¹²³⁴,Loukil Ahmed¹³^ORCID,Fellag Mustapha¹³,Diallo Boukary⁵,Ouedraogo Abdoul Salam⁴,Godreuil Sylvain²,Drancourt Michel³

Affiliation:

1. IHU Méditerranée Infection, Marseille, France

2. Université de Montpellier, IRD, MIVEGEC, Montpellier, France

3. Aix-Marseille-University, IRD, MEPHI, IHU Méditerranée Infection, Marseille, France

4. Service de Bactériologie-Virologie, Centre Hospitalier Universitaire Souro Sanou, Bobo-Dioulasso, Burkina Faso

5. Service de Dermatologie-Vénéréologie, Centre Hospitalier Universitaire Souro Sanou, Bobo-Dioulasso, Burkina Faso

Abstract

Leprosy is caused by Mycobacterium leprae , and it remains underdiagnosed in Burkina Faso. We investigated the use of fluorescent in situ hybridization (FISH) for detecting M. leprae in 27 skin samples (skin biopsy samples, slit skin samples, and skin lesion swabs) collected from 21 patients from Burkina Faso and three from Côte d’Ivoire who were suspected of having cutaneous leprosy. In all seven Ziehl-Neelsen-positive skin samples (four skin biopsy samples and three skin swabs collected from the same patient), FISH specifically identified M. leprae , including one FISH-positive skin biopsy sample that remained negative after testing with PCR targeting the rpoB gene and with the GenoType LepraeDR assay.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02130-19

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