Affiliation:
1. Department of Molecular Cell Biology
2. Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands
Abstract
ABSTRACT
Schistosoma
sp. circular anodic antigen (CAA) serum concentrations reflect actual worm burden in a patient and are a valuable tool for population screening and epidemiological research. However, for the diagnosis of individual imported schistosomiasis cases, the current enzyme-linked immunosorbent assay (ELISA) lacks sensitivity and robustness. Therefore, a lateral flow (LF) assay was developed to test CAA in serum for individual diagnosis of imported active schistosome infections. Application of fluorescent submicron-sized up-converting phosphor technology (UPT) reporter particles increased analytical sensitivity compared to that of the standard ELISA method. Evaluation of the UPT-LF test with a selection of 40 characterized epidemiologic samples indicated a good correlation between signal intensity and infection intensity. Subsequently, the UPT-LF assay was applied to 166 serum samples of Dutch residents (immigrants and travelers) suspected of schistosomiasis, a case in which group routine antibody detection frequently fails straightforward diagnosis. The UPT-LF assay identified 36 CAA-positive samples, compared to 15 detected by CAA-ELISA. In conclusion, the UPT-LF assay is a low-complexity test with higher sensitivity than the CAA-ELISA, well suited for laboratory diagnosis of individual active
Schistosoma
infections.
Publisher
American Society for Microbiology
Reference29 articles.
1. Bierman, W. F., J. C. Wetsteyn, and T. van Gool. 2005. Presentation and diagnosis of imported schistosomiasis: relevance of eosinophilia, microscopy for ova, and serology. J. Travel Med.12:9-13.
2. Bottieau, E., J. Clerinx, M. R. de Vega, E. Van den Enden, R. Colebunders, M. Van Esbroeck, T. Vervoort, A. Van Gompel, and J. Van den Ende. 2006. Imported Katayama fever: clinical and biological features at presentation and during treatment. J. Infect.52:339-345.
3. Corachan, M. 2002. Schistosomiasis and international travel. Clin. Infect. Dis.35:446-450.
4. Corstjens, P., M. Zuiderwijk, A. Brink, S. Li, H. Feindt, R. S. Niedbala, and H. Tanke. 2001. Use of up-converting phosphor reporters in lateral-flow assays to detect specific nucleic acid sequences: a rapid, sensitive DNA test to identify human papillomavirus type 16 infection. Clin. Chem.47:1885-1893.
5. Corstjens, P. L., S. Li, M. Zuiderwijk, K. Kardos, W. R. Abrams, R. S. Niedbala, and H. J. Tanke. 2005. Infrared up-converting phosphors for bioassays. IEE Proc. Nanobiotechnol.152:64-72.