Occurrence of alternatively spliced leader-delta onc-poly(A) transcripts in chicken neuroretina cells infected with Rous-associated virus type 1: implication in transduction of the c-mil/c-raf and c-Rmil/B-raf oncogenes

Author:

Felder M P1,Laugier D1,Eychene A1,Calothy G1,Marx M1

Affiliation:

1. Institut Curie, Centre Universitaire, Orsay, France.

Abstract

We previously reported that serial passaging of Rous-associated virus type 1 in nondividing chicken embryo neuroretina cells leads to reproducible generation of acutely mitogenic retroviruses that transduced the catalytic domain of c-mil/c-raf or c-Rmil/B-raf. On the basis of structural analysis of several retroviruses, we proposed that the early step of oncogene transduction is the constitution of alternatively spliced leader-delta onc-poly(A) transcripts. Here, we show that neuroretina cells do synthesize hybrid leader-delta mil and leader-delta Rmil RNAs and that these RNAs exhibit mitogenic properties and serve as templates for the generation of transducing retorviruses.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference25 articles.

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3. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease;Chirgwin J. M.;Biochemistry,1979

4. Quail neuroretina c-Rmil(Braf) proto-oncogene cDNAs encode two proteins of 93.5 and 95 kDa resulting from alternative splicing;Eychene A.;Oncogene,1992

5. Molecular and biological properties of c-mil transducing retroviruses generated during passage of Rous-associated virus type 1 in chicken neuroretina cells;Eychene A.;J. Virol.,1990

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