Enhancement of Glutamine Utilization in Bacillus subtilis through the GlnK-GlnL Two-Component Regulatory System-Reference-Cited by-同舟云学术

Enhancement of Glutamine Utilization in Bacillus subtilis through the GlnK-GlnL Two-Component Regulatory System

Published:2005-07 Issue:14 Volume:187 Page:4813-4821
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Satomura Takenori¹,Shimura Daisuke²,Asai Kei²,Sadaie Yoshito²,Hirooka Kazutake¹,Fujita Yasutaro¹

Affiliation:

1. Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama 729-0292

2. Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, Saitama 338-8570, Japan

Abstract

ABSTRACT During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates the expression of the glsA-glnT (formerly ybgJ-ybgH ) operon in response to glutamine in the culture medium on Northern analysis. As a result of gel retardation and DNase I footprinting analyses, we found that the GlnL protein interacts with a region (bases −13 to −56; +1 is the transcription initiation base determined on primer extension analysis of glsA - glnT ) in which a direct repeat, TTTTGTN4TTTTGT, is present. Furthermore, the glsA and glnT genes were biochemically verified to encode glutaminase and glutamine transporter, respectively.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.187.14.4813-4821.2005

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