Abstract
An assay for bovine interferons has been developed using the porcine cell line IB-RS-2 and a bovine enterovirus, CBV-D, as challenge virus. The method is based on estimation of cytopathic effect measured by uptake of neutral red. Teh assay is simple, sensitive, and reproducible. A comparative test of different viruses in IB-RS-2 cells and secondary calf kidney cells revealed that the sensitivity of a virus to interferon can vary up to 1,000-fold in the two cell systems. Vesicular stomatitis virus was found to be rather insensitive to interferon in IB-RS-2 cells.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference24 articles.
1. Inhibitors of foot- and mouth disease virus. I. Effect of EDTA, temperature, and interferon on the viral growth cycle;Ahl R.;Arch. Gesamte Virusforsch.,1974
2. Die lokale Interferonbildung im Respirations- und Genitaltrakt nach experimenteller Infektion mit Rhinotracheitis (IIBR)- und Bliaschenausachiag (IPV)-Virus;Ahl R.;Dtsch. Tieraerztl. Wochenschr.,1971
3. Semi-micro, dye-binding assay for rabbit interferon;Armstrong J. A.;Appl. Microbiol.,1971
4. C-type virus particles in pig kidney cell lines;Armstrong J. A.;J. Gen. Virol.,1971
5. A micro assay for mouse and human interferon;Dahl H.;Acta Pathol. Microbiol. Scand. Sect. B,1972
Cited by
33 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献