Electrostatic Interactions Drive the Self-Assembly and the Transcription Activity of the Pseudomonas Phage ϕ6 Procapsid-Reference-Cited by-同舟云学术

Electrostatic Interactions Drive the Self-Assembly and the Transcription Activity of the Pseudomonas Phage ϕ6 Procapsid

Published:2014-06-15 Issue:12 Volume:88 Page:7112-7116
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Sun Xiaoyu¹²,Bamford Dennis H.¹²,Poranen Minna M.¹

Affiliation:

1. Department of Biosciences, University of Helsinki, Vikki Biocenter, Helsinki, Finland

2. Institute of Biotechnology, University of Helsinki, Viikki Biocenter, Helsinki, Finland

Abstract

ABSTRACT Assembly of an empty procapsid is a crucial step in the formation of many complex viruses. Here, we used the self-assembly system of the double-stranded RNA bacteriophage ϕ6 to study the role of electrostatic interactions in a scaffolding-independent procapsid assembly pathway. We demonstrate that ϕ6 procapsid assembly is sensitive to salt at both the nucleation and postnucleation steps. Furthermore, we observed that the salt sensitivity of ϕ6 procapsid-directed transcription is reversible.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00467-14

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