Affiliation:
1. Department of Biology, Southwest Missouri State University, 901 S. National, Springfield, Missouri 65804
Abstract
A hydrogen gas (H
2
)-producing strain of
Ectothiorhodospira vacuolata
isolated from Soap Lake, Washington, possessed nitrogenase activity. Increasing evolution of H
2
with decreasing ammonium chloride concentrations provided evidence that nitrogenase was the catalyst in gas production. Cells were grown in a mineral medium plus 0.2% acetate with sodium sulfide as an electron donor. Factors increasing H
2
production included addition of reduced carbon compounds such as propionate and succinate, increased reducing power by increasing sodium sulfide concentrations, and increased energy charge (ATP) by increasing light intensity.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
10 articles.
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