Quantitative Proteome Profiling Reveals Cellobiose-Dependent Protein Processing and Export Pathways for the Lignocellulolytic Response in Neurospora crassa-Reference-Cited by-同舟云学术

Quantitative Proteome Profiling Reveals Cellobiose-Dependent Protein Processing and Export Pathways for the Lignocellulolytic Response in Neurospora crassa

Published:2020-07-20 Issue:15 Volume:86 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Liu Dan¹²,Liu Yisong¹²,Zhang Duoduo¹²,Chen Xiaoting¹²,Liu Qian¹²,Xiong Bentao¹²,Zhang Lihui¹²,Wei Linfang¹²,Wang Yifan¹²,Fang Hao¹²^ORCID,Liesche Johannes¹²,Wei Yahong¹³²,Glass N. Louise⁴⁵^ORCID,Hao Zhiqi⁶,Chen Shaolin¹³²^ORCID

Affiliation:

1. Biomass Energy Center for Arid and Semi-Arid Lands, Northwest A&F University, Yangling, People’s Republic of China

2. College of Life Sciences, Northwest A&F University, Yangling, People’s Republic of China

3. Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, Northwest A&F University, Yangling, People’s Republic of China

4. Department of Plant and Microbial Biology, University of California at Berkeley, Berkeley, California, USA

5. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

6. Thermo Fisher Scientific, San Jose, California, USA

Abstract

Lignocellulases are important industrial enzymes for sustainable production of biofuels and bio-products. Insoluble cellulose has been commonly used to induce the production of lignocellulases in filamentous fungi, which causes a difficult fermentation operation and enzyme loss due to adsorption to cellulose. The disadvantages can be overcome by using soluble inducers, such as the disaccharide cellobiose. Quantitative proteome profiling of the model filamentous fungus Neurospora crassa revealed cellobiose-dependent pathways for cellulase production, including protein processing and export. A protein (CWH43) potentially involved in protein processing was found to be a positive regulator of lignocellulase production. The cellobiose-dependent mechanisms provide new opportunities to improve the production of lignocellulases in filamentous fungi.

Funder

U.S. Department of Energy

Ministry of Science and Technology of the People's Republic of China

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00653-20

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1. Fungal Cellulases