Author:
Musial C E,Arrowood M J,Sterling C R,Gerba C P
Abstract
Members of the genus Cryptosporidium are protozoan parasites that cause gastroenteritis in humans and animals and appear to be spread largely by the fecal-oral route. A method was developed for the concentration and detection of Cryptosporidium oocysts in water to assess their occurrence in the environment and potential for waterborne disease transmission. This method was developed by using spun polypropylene cartridge filters. Optimal conditions for concentration, filter elution, filter porosity, and detection were determined. Fluoresceinated monoclonal antibodies were used for oocyst detection. Experiments also were conducted to study the effect of flow rate, low oocyst numbers, and the addition of detergents on recovery and retention of oocysts. The method that was developed was sensitive enough to detect oocysts at levels of less than 1 per liter. Using this method, we isolated Cryptosporidium oocysts from secondarily treated sewage.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference20 articles.
1. Bitton G. 1980. Introduction to environmental virology. John Wiley & Sons Inc. New York.
2. Current W. L. 1984. Cryptosporidium and cryptosporidiosis p. 355-373. In M. S. Gottleib and J. D. Groopman (ed.) Acquired immune deficiency syndrome. Alan R. Liss Inc. New York.
3. Human cryptosporidiosis in immunocompetent and immunodeficient persons: studies of an outbreak and experimental transmission;Current W. L.;N. Engl. J. Med.,1983
4. A waterborne outbreak of cryptosporidiosis in normal hosts;D'Antonio R. G.;Ann. Intern. Med.,1985
5. Freifelder D. 1982. Physical biochemistry: applications to biochemistry and molecular biology 2nd ed. W. H. Freeman and Co. San Francisco.
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