Tetrazolium Reduction-Malachite Green Method for Assessing the Viability of Filamentous Bacteria in Activated Sludge-Reference-Cited by-同舟云学术

Tetrazolium Reduction-Malachite Green Method for Assessing the Viability of Filamentous Bacteria in Activated Sludge

Published:1982-04 Issue:4 Volume:43 Page:964-966
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bitton Gabriel¹,Koopman Ben¹

Affiliation:

1. Department of Environmental Engineering Sciences, University of Florida, Gainesville, Florida 32611

Abstract

A method was developed to assess the activity of filamentous bacteria in activated sludge. It involves the incubation of activated sludge with 2( p -iodophenyl)-3-( p -nitrophenyl)-5-phenyl tetrazolium chloride followed by staining with malachite green. Both cells and 2( p -iodophenyl)-3-( p -nitrophenyl)-5-phenyl tetrazolium chloride-formazan crystals can be observed in prepared specimens by using bright-field microscopy. This procedure allowed us to distinguish between inactive and actively metabolizing filaments after chlorine application to control the bulking of activated sludge.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.43.4.964-966.1982

Reference16 articles.

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5. Some experiences with the chlorination of activated sludge for the fight against bulking sludge in the Berlin-Ruhleben treatment plant;Frenzel H. J.;Prog. Water Technol.,1977

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