Affiliation:
1. Department of Biology, Yale University, New Haven, Connecticut 06520
Abstract
Several mutant strains of
Pseudomonas putida
, selected on the basis of their inability to grow at the expense of benzoate, have been shown to be unable to form inducibly both muconate lactonizing enzyme and muconolactone isomerase. A secondary mutant strain derived from one of these pleiotropically negative strains forms these two enzymes and, in addition, catechol oxygenase in the absence of inducer. This constitutive mutant strain was used as a donor in transductionally mediated two-point crosses to determine the order of point mutations within the structural genes for muconate lactonizing enzyme and muconolactone isomerase (the
catB
and
catC
genes, respectively). The gene order conformed precisely with the one that has been established by deletion mapping.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference15 articles.
1. Adams M. H. 1959. Bacteriophages. Interscience Publishers Inc. New York.
2. Transduction and the clustering of genes in fluorescent pseudomonads;Chakrabarty A. M.;Proc. Nat. Acad. Sci. U.S.A.,1968
3. Positive control of enzyme synthesis by gene C in the Larabinose system;Englesberg E.;J. Bacteriol.,1965
4. An analysis of "revertants;Englesberg E.;J. Mol. Biol.,1969
5. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type;Hegeman G. D.;J. Bacteriol.,1966
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