tRNAs Are Stable After All: Pitfalls in Quantification of tRNA from Starved Escherichia coli Cultures Exposed by Validation of RNA Purification Methods-Reference-Cited by-同舟云学术

tRNAs Are Stable After All: Pitfalls in Quantification of tRNA from Starved Escherichia coli Cultures Exposed by Validation of RNA Purification Methods

Published:2023-02-28 Issue:1 Volume:14 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Prossliner Thomas¹,Agrawal Shreya¹,Heidemann Ditte F.¹,Sørensen Michael A.¹,Svenningsen Sine L.¹^ORCID

Affiliation:

1. Department of Biology, University of Copenhagen, Copenhagen, Denmark

Abstract

We show that E. coli tRNAs are remarkably stable during several days of nutrient starvation, although rRNA is degraded extensively under these conditions. The levels of these two major RNA classes are considered to be strongly coregulated at the level of transcription.

Funder

Danmarks Frie Forskningsfond

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mbio.02805-22

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