Genomic and Biochemical Analysis of N Glycosylation in the Mushroom-Forming Basidiomycete Schizophyllum commune

Author:

Berends Elsa12,Ohm Robin A.1,de Jong Jan F.1,Rouwendal Gerard3,Wösten Han A. B.1,Lugones Luis G.1,Bosch Dirk23

Affiliation:

1. Molecular Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

2. Membrane Enzymology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

3. Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands

Abstract

ABSTRACT N-linked glycans of Schizophyllum commune consist of Man 5-9 GlcNAc 2 structures. Lack of further glycan maturation is explained by the absence of genes encoding such functions in this and other homobasidiomycetes. N-linked glycans in vegetative mycelium and fruiting bodies of S. commune are mainly Man 7 GlcNAc 2 and Man 5 GlcNAc 2 , respectively, suggesting more efficient mannose trimming in the mushroom.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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