LDB1 Enforces Stability on Direct and Indirect Oncoprotein Partners in Leukemia-Reference-Cited by-同舟云学术

LDB1 Enforces Stability on Direct and Indirect Oncoprotein Partners in Leukemia

Published:2020-05-28 Issue:12 Volume:40 Page:
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Layer Justin H.¹,Christy Michael¹,Placek Lindsey²,Unutmaz Derya²,Guo Yan³,Davé Utpal P.¹⁴

Affiliation:

1. Division of Hematology/Oncology, Indiana University School of Medicine, Indianapolis, Indiana, USA

2. The Jackson Laboratory for Genomic Medicine, Farmington, Connecticut, USA

3. University of New Mexico Cancer Center, Albuquerque, New Mexico, USA

4. Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA

Abstract

The LMO2/LDB1 macromolecular complex is critical in hematopoietic stem and progenitor cell specification and in the development of acute leukemia. This complex is comprised of core subunits of LMO2 and LDB1 as well as s ingle- s tranded DNA- b inding p rotein (SSBP) cofactors and DNA-binding b asic h elix- l oop- h elix (bHLH) and GATA transcription factors. We analyzed the steady-state abundance and kinetic stability of LMO2 and its partners via Halo protein tagging in conjunction with variant proteins deficient in binding their respective direct protein partners.

Funder

HHS | NIH | National Cancer Institute

Indiana University

Biomedical Laboratory Research and Development, VA Office of Research and Development

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.00652-19

Reference65 articles.

1. SnapShot: Hematopoiesis