LABELING PROCEDURES EMPLOYING CRYSTALLINE FLUORESCEIN ISOTHIOCYANATE-Reference-Cited by-同舟云学术

LABELING PROCEDURES EMPLOYING CRYSTALLINE FLUORESCEIN ISOTHIOCYANATE

Published:1961-10 Issue:4 Volume:82 Page:534-537
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Griffin Charles W.¹²,Carski Theodore R.¹²,Warner George S.¹²

Affiliation:

1. Research and Development Laboratory, Baltimore Biological Laboratory, and Biological Research Laboratories, Hynson, Westcott

2. Dunning, Inc., Baltimore, Maryland

Abstract

Griffin, Charles W. (Baltimore Biological Laboratory, Baltimore, Md.), Theodore R. Carski, and George S. Warner . Labeling procedures employing crystalline fluorescein isothiocyanate. J. Bacteriol. 82: 534–537. 1961.—The effect of altering the ratio of dye to protein in the labeling of three immune globulins with crystalline fluorescein isothiocyanate was the purpose of the study. Direct staining of group A streptococci and rabies-infected mouse brain, and indirect staining of Treponema pallidum (Nichols strain) comprised the three immunological systems selected for investigation. Maximal specific staining and minimal nonspecific background staining were demonstrated by the use of low dye-to-protein labeling ratios.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.82.4.534-537.1961

Reference12 articles.

1. Immunofluorescent staining: the fluorescent antibody method;BEUTNER E. H.;Bacteriol. Rev.,1961

2. Fluorescent protein tracers: the unreacted fluorescent material in fluorescein conjugates and studies of conjugates with other green fluorochromes;CHADWICK C. S.;Immunology,1960

3. Localization of antigen in tissue cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody;COONS A. H.;J. Exptl. Med.,1950

4. Electrophoresis of fluorescent antibody;CURTAIN C. C.;Nature,1958

5. A fluorescent test for treponemal antibodies;DEACON W. E.;Proc. Soc. Exptl. Biol. Med.,1957

Cited by 27 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Glycoprotein labeling with click chemistry (GLCC) and carbohydrate detection;Carbohydrate Research;2015-08

2. Colloidal synthesis and blue based multicolor upconversion emissions of size and composition controlled monodisperse hexagonal NaYF4 : Yb,Tm nanocrystals;Nanoscale;2010

3. SPECIFIC AND NON-SPECIFIC IMMUNOFLUORESCENT REACTIONS SHOWN BY DIFFERENT PREPARATIONS OF FITC-LABELLED RABBIT ANTI-GONOCOCCAL GLOBULIN;Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology;2009-08-15

4. EXPERIMENTAL STUDIES ON BOVINE TICK-BORNE FEVER;Acta Pathologica Microbiologica Scandinavica;2009-08-15

5. Vibrationally-Electronically Doubly Resonant Sum-Frequency Generation Spectroscopy of Organic Thin Films;The Review of Laser Engineering;2009