Differentiation between mouse-virulent and -avirulent strains of Toxoplasma gondii by a monoclonal antibody recognizing a 27-kilodalton antigen

Author:

Bohne W1,Gross U1,Heesemann J1

Affiliation:

1. Institute of Hygiene and Microbiology, University of Würzburg, Germany.

Abstract

Using a murine monoclonal antibody, we were able to differentiate between mouse-virulent and -avirulent strains of Toxoplasma gondii. Monoclonal antibody TB6G5 was reactive with eight clinical mouse-avirulent isolates but not with mouse-virulent laboratory strains RH and BK. The antibody-reactive antigen was identified by indirect immunofluorescence and immunoblot as a 27-kDa cytoplasmic protein expressed by tachyzoites as well as by bradyzoites.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference18 articles.

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3. Protection of mice from fatal Toaxoplasma gondii infection by immunization with p30 antigen in liposomes;Bulow R.;J. Immunol.,1991

4. Toxoplasma gondii: characterisation and localisation of antigens secreted from tachyzoites;Charif H.;Exp. Parasitol.,1990

5. Isoenzymic characterization of seven strains of Toxoplasma gondii by isoelectrofocusing in polyacrylamide gels;Darde M. L.;Am. J. Trop. Med. Hyg.,1989

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