Coryneform bacteria isolated from middle ear fluid

Author:

Simonet M1,De Briel D1,Boucot I1,Minck R1,Veron M1

Affiliation:

1. Laboratoire de Microbiologie, Faculté de Médecine Necker-Enfants Malades, Paris, France.

Abstract

Nineteen strains of facultatively anaerobic gram-positive rods isolated in pure culture from middle ear fluids were identified. All effusions were collected by tympanocentesis in children with acute otitis media. Identification of microorganisms to the genus level was done by studying the cell wall composition. Sixteen strains contain meso-diaminopimelic acid and arabinogalactan polymer but lack mycolic acids; therefore, these strains do not belong to a previously described taxon. Because of similarities with Corynebacterium afermentans (Centers for Disease Control group ANF-1), we temporarily classified these mycolateless strains ANF-1 like. Isolation of these microorganisms in pure culture from middle ear fluids collected by tympanocentesis is a strong argument for their involvement in acute otitis media.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference11 articles.

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5. Gehanno P. B. Cohen P. Berche and I. Boucot. 1991. Epidemiology of acute otitis media in children in France between 1987-1991. Recent advances in otitis media abstr. 149. Proceedings of the 5th International Symposium of the Ohio State University and the Deafness Research Foundation Fort Lauderdale Fla.

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