Precise Adsorption Method for Measuring the Percentage of Dead Bacterial Cells

Author:

Vairo Marina L. R.1,Borzani Walter1

Affiliation:

1. Department of Chemical Engineering, Escola Politecnica, University of São Paulo, São Paulo, Brazil

Abstract

It was observed that Freundlich's law is obeyed when methylene blue is adsorbed from different aqueous solutions by mixtures of dead and live bacterial cells. A simple and precise colorimetric method was developed for determining the percentage of dead cells.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference9 articles.

1. Quantitative adsorption of methylene blue by dead yeast cells;BORZANI W.;J. Bacteriol.,1958

2. Quantitative adsorption of crystal violet by dead microorganisms;BORZANI W.;J. Bacteriol.,1959

3. Adsorption method for determining the percentage of dead cells in suspensions of Sarcina Iutea;BORZANI W.;J. Bacteriol.,1960

4. Adsorption of methylene blue as a means of determining cell concentration of dead bacteria in suspensions;BORZANI W.;Stain Technol.,1960

5. JOERGENsEN A. 1948. Microorganisms and fermentation p. 223. Charles Griffin and Co. Ltd. London.

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