Affiliation:
1. Fort Detrick, Frederick, Maryland 21701
Abstract
Simple and efficient methods for concentrating Rift Valley fever (RVF) virus and chikungunya (CHIK) virus are described. Ammonium sulfate, potassium sulfate, or alcohol was used as a precipitating agent and the precipitate was resuspended to volumes suitable for further processing and purification. The methods permitted concentration of live RVF virus and CHIK virus about 100-fold with negligible losses of virus. RVF virus retained a high level of infectivity with potassium aluminum sulfate and alcohol, but CHIK virus retained a higher infectivity level with ammonium sulfate than with potassium aluminum sulfate. The data indicate that serum plays an important role in the concentration of both viruses, at least when the sulfate methods are used.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference21 articles.
1. Some characteristics of plaque variants of Rift Valley fever virus;Boyle J. J.;Amer. J. Vet. Res.,1967
2. Controlled pH and oxidation-reduction potential with a new glass tissue culture fermentor;Daniels W. F.;Biotechnol. Bioeng.,1965
3. Physical properties of the viruses of Newcastle disease, fowl plague, and mumps;Elford W. J.;Brit. J. Exp. Pathol.,1948
4. Finney D. J. 1952. Statistical methods in biological assay. Hafner Publishing Co. New York.
5. Column purification of influenza virus;Frimmhagen L. H.;Virology,1959
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