Conversion of Lactobacillus pentosus d -Lactate Dehydrogenase to a d -Hydroxyisocaproate Dehydrogenase through a Single Amino Acid Replacement-Reference-Cited by-同舟云学术

Conversion of Lactobacillus pentosus d -Lactate Dehydrogenase to a d -Hydroxyisocaproate Dehydrogenase through a Single Amino Acid Replacement

Published:2003-08-15 Issue:16 Volume:185 Page:5023-5026
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Tokuda Chizuka¹,Ishikura Yoshiro¹,Shigematsu Mayu¹,Mutoh Hiroyuki¹,Tsuzuki Shino¹,Nakahira Yusaku¹,Tamura Yusuke¹,Shinoda Takeshi¹,Arai Kazuhito¹,Takahashi O¹,Taguchi Hayao¹

Affiliation:

1. Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Noda, Chiba 278-8510, Japan

Abstract

ABSTRACT The single amino acid replacement of Tyr52 with Leu drastically increased the activity of Lactobacillus pentosus NAD-dependent d -lactate dehydrogenase toward larger aliphatic or aromatic 2-ketoacid substrates by 3 or 4 orders of magnitude and decreased the activity toward pyruvate by about 30-fold, converting the enzyme into a highly active d -2-hydroxyisocaproate dehydrogenase.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.185.16.5023-5026.2003

Reference38 articles.

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