Affiliation:
1. Unité de Génétique des Génomes Bactériens
2. Génopole-Plateau Protéomique
3. Laboratoire des Régulations Transcriptionnelles, Institut Pasteur, 75724 Paris Cedex 15, France
Abstract
ABSTRACT
The cAMP-catabolite activator protein (CAP) complex is a pleiotropic regulator that regulates a vast number of
Escherichia coli
genes, including those involved in carbon metabolism. We identified two new targets of this complex:
argG
, which encodes the arginosuccinate synthase involved in the arginine biosynthetic pathway, and
metY
, which encodes one of the two methionine tRNA initiators, tRNAf2Met. The cAMP-CAP complex activates
argG
transcription and inhibits
metY
transcription from the same DNA position. We also show that ArgR, the specific repressor of the arginine biosynthetic pathway, together with its arginine cofactor, acts on the regulation of
metY
mediated by CAP. The regulation of the two divergent promoters is thus simultaneously controlled not only by the cAMP-CAP complex, a global regulator, but also by a specific regulator of arginine metabolism, suggesting a previously unsuspected link between carbon metabolism and translation initiation.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
24 articles.
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