Ultrafast Assessment of the Presence of a High-Risk Mycobacterium tuberculosis Strain in a Population-Reference-Cited by-同舟云学术

Ultrafast Assessment of the Presence of a High-Risk Mycobacterium tuberculosis Strain in a Population

Published:2016-03 Issue:3 Volume:54 Page:779-781
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Pérez-Lago Laura¹²³,Herranz Marta¹²³,Comas Iñaki⁴⁵,Ruiz-Serrano María Jesús¹²³,López Roa Paula⁶,Bouza Emilio¹²³⁷,García-de-Viedma Darío¹²³⁸

Affiliation:

1. Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain

2. Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

3. CIBER Enfermedades Respiratorias, CIBERES, Spain

4. Genomics and Health Unit, FISABIO Public Health, Valencia, Spain

5. CIBER Salud Pública CIBERESP, Spain

6. Servicio de Microbiología, Hospital General Universitario Doce de Octubre, Instituto de Investigación Hospital 12 de Octubre, Madrid, Spain

7. Departamento de Medicina, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain

8. CEI Campus Moncloa, UCM-UPM, Madrid, Spain

Abstract

ABSTRACT A persistent 8-year infection by a Beijing Mycobacterium tuberculosis strain from a previous outbreak after importation from West Africa obliged us to investigate secondary cases. We developed a multiplex PCR method based on whole-genome sequencing to target strain-specific single nucleotide polymorphisms (SNPs). In 1 week, we analyzed 868 isolates stored over 6 years. Only 2 cases (immigrants from Guinea Conakry) harbored the strain, which ruled out transmission—despite opportunities—and challenged some of the advantages associated with Beijing strains.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02851-15

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