Propagation and primary isolation of papovavirus JC in epithelial cells derived from human urine

Author:

Beckmann A M,Shah K V,Padgett B L

Abstract

Human papovavirus JC, previously passaged in amnion cells or in primary human fetal glial cells, replicated efficiently in urine-derived epithelial cells. Primary isolation of the virus from brain extracts was possible in urine-derived cells, but these cells were not as sensitive as primary human fetal glial cells for this purpose. Primary isolations of human papovavirus JC from urine sediments of renal transplant patients were made in urine-derived cells.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference11 articles.

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2. Fareed G. C. K. K. Takemoto and M. A. Gimbrone Jr. 1978. Interaction of simian virus 40 and human papovaviruses BK and JC with human vascular endothelial cells p. 427-431. In D. Schlessinger (ed.) Microbiology-1978. American Society of Microbiology Washington D.C. NOTES 777

3. Human epithelial cells cultured from urine: growth properties and keratin staining;Felix J. S.;Vitro,1980

4. Characterization of JC papovavirus adapted to growth in human embryonic kidney cells;Miyamura T.;J. Virol.,1980

5. Comparison of JC and BK human papovaviruses with simian virus 40: restriction endonuclease digestion and gel electrophoresis of resultant fragments;Osborn J. E.;J. Virol.,1974

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