Genotypic Antiretroviral Resistance Testing for Human Immunodeficiency Virus Type 1 Integrase Inhibitors by Use of the TruGene Sequencing System-Reference-Cited by-同舟云学术

Genotypic Antiretroviral Resistance Testing for Human Immunodeficiency Virus Type 1 Integrase Inhibitors by Use of the TruGene Sequencing System

Published:2008-12 Issue:12 Volume:46 Page:4087-4090
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Paar Christian¹,Palmetshofer Christina¹,Flieger Karin²,Geit Maria³,Kaiser Rolf⁴,Stekel Herbert¹,Berg Jörg¹

Affiliation:

1. Institute of Laboratory Medicine, General Hospital Linz, Krankenhausstrasse 9, A-4020 Linz, Austria

2. Siemens Medical Solutions Diagnostics, Hohe Strasse 4-8, D-61231 Bad Nauheim, Germany

3. Department of Dermatology, General Hospital Linz, Krankenhausstrasse 9, A-4020 Linz, Austria

4. Institute of Virology, University of Cologne, Fürst-Pückler-Strasse, D-5650935 Cologne, Germany

Abstract

ABSTRACT A sequencing assay for detection of mutations conferring resistance to human immunodeficiency virus type 1 (HIV-1) integrase inhibitors raltegravir and elvitegravir was developed using the automated TruGene sequencing system. The assay returned clear sequencing results for samples with ≥500 RNA copies/ml for mutation detection and HIV-1 subtyping across a spectrum of HIV-1 subtypes.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01246-08

Reference16 articles.

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