Efficient Quantification of HIV-1 in Heparin Plasma Spiked with Cultured HIV-1 by the Roche Cobas TaqMan and Abbott RealTime HIV-1 Tests-Reference-Cited by-同舟云学术

Efficient Quantification of HIV-1 in Heparin Plasma Spiked with Cultured HIV-1 by the Roche Cobas TaqMan and Abbott RealTime HIV-1 Tests

Published:2012-08 Issue:8 Volume:50 Page:2804-2806
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Jagodzinski Linda L.¹,Weston Holly R.²,Liu Ying²,O'Connell Robert J.¹,Peel Sheila A.¹

Affiliation:

1. U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA

2. U.S. Military HIV Research Program, Henry M. Jackson Foundation, Rockville, Maryland, USA

Abstract

ABSTRACT The current automated real-time HIV-1 viral load assays, the Roche Cobas AmpliPrep/Cobas TaqMan test and the Abbott RealTime test, are FDA cleared for use with EDTA plasma. We show that both real-time reverse transcription-PCR (RT-PCR) tests reliably quantify HIV-1 RNA in heparin plasma specimens spiked with HIV-1 isolate MN.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00706-12

Reference16 articles.

1. Abbott Molecular Inc. 2009. Abbott RealTi me HIV-1 assay package insert. REF 6L18. 51-602146/R4. Abbott Molecular Inc. Des Plaines IL.

2. Rapid and simple method for purification of nucleic acids

3. Optimization of Specimen-Handling Procedures for Accurate Quantitation of Levels of Human Immunodeficiency Virus RNA in Plasma by Reverse Transcriptase PCR

4. An optimized sensitive method for quantitation of DNA/RNA viruses in heparinized and cyropreserved plasma;Ding M;J. Virol. Methods,2011

5. Immunologic and virologic events in early HIV infection predict subsequent rate of progression;Ganesan A;J. Infect. Dis.,2010

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A Rapid and Sensitive Detection of HIV-1 with a One-Pot Two-Stage Reverse Transcription Recombinase Aided Real-Time PCR Assay;Tropical Medicine and Infectious Disease;2023-02-06

2. Specimen Requirements Selection, Collection, Transport, and Processing;Clinical Virology Manual;2016-04-15