Change in Cofactor Specificity of Oxidoreductases by Adaptive Evolution of an Escherichia coli NADPH-Auxotrophic Strain-Reference-Cited by-同舟云学术

Change in Cofactor Specificity of Oxidoreductases by Adaptive Evolution of an Escherichia coli NADPH-Auxotrophic Strain

Published:2021-08-31 Issue:4 Volume:12 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Bouzon Madeleine¹,Döring Volker¹,Dubois Ivan¹,Berger Anne¹,Stoffel Gabriele M. M.²,Calzadiaz Ramirez Liliana³,Meyer Sophia N.³,Fouré Marion¹,Roche David¹,Perret Alain¹,Erb Tobias J.²⁴,Bar-Even Arren³^ORCID,Lindner Steffen N.³^ORCID

Affiliation:

1. Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, Evry-Courcouronnes, France

2. Max Planck Institute of Terrestrial Microbiology, Marburg, Germany

3. Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

4. LOEWE Research Center for Synthetic Microbiology (SYNMIKRO), Marburg, Germany

Abstract

In the cell, NAD(H) and NADP(H) cofactors have different functions. The former mainly accepts electrons from catabolic reactions and carries them to respiration, while the latter provides reducing power for anabolism.

Funder

Max Planck Society

CEA Genoscope

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mBio.00329-21

Reference28 articles.

1. A General Tool for Engineering the NAD/NADP Cofactor Preference of Oxidoreductases

2. Protein Engineering for Nicotinamide Coenzyme Specificity in Oxidoreductases: Attempts and Challenges