Improving the Precision of Base Editing by Bubble Hairpin Single Guide RNA-Reference-Cited by-同舟云学术

Improving the Precision of Base Editing by Bubble Hairpin Single Guide RNA

Published:2021-04-27 Issue:2 Volume:12 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Hu Zhiwei¹,Wang Yannan¹,Liu Qian¹,Qiu Yan¹,Zhong Zhiyu¹,Li Kangdi²,Li Wenhua²,Deng Zixin¹,Sun Yuhui¹

Affiliation:

1. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), School of Pharmaceutical Sciences, Wuhan University, Wuhan, People’s Republic of China

2. Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of China

Abstract

Base editors are double-strand-break (DSB)-free genome editing tools and have been widely used in diverse living systems. However, it is reported that these tools can cause substantial off-target editing.

Funder

The National Key R&D Program of China

The National Natural Science Foundation of China

The Funds for International Cooperation and Exchange of the National Naturak Science Foundation of China

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mBio.00342-21

Reference28 articles.

1. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage