Generation of Mature DENVs via Genetic Modification and Directed Evolution-Reference-Cited by-同舟云学术

Generation of Mature DENVs via Genetic Modification and Directed Evolution

Published:2022-06-28 Issue:3 Volume:13 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Tse Longping V.¹^ORCID,Meganck Rita M.¹,Dong Stephanie¹,Adams Lily E.¹²^ORCID,White Laura J.²,Mallory Michael L.¹,Jadi Ramesh²,de Silva Aravinda M.²,Baric Ralph S.¹²^ORCID

Affiliation:

1. Department of Epidemiology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

2. Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

Abstract

Mature DENVs represent the dominant form in vivo and are the target for vaccine development. Here, we used multiple strategies, including protein engineering and natural and directed evolution to generate DENV1, -2, -3, and -4 variants that are highly mature without compromising replication efficiency compared to the parental strains.

Funder

NIH

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mbio.00386-22

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