Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase-Reference-Cited by-同舟云学术

Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase

Published:2023-04-25 Issue:2 Volume:14 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Fan Li¹²,Zhang Wenqing¹,Rybchuk Josephine²³,Luo Yu²,Xiao Wei¹²^ORCID

Affiliation:

1. Beijing Key Laboratory of DNA Damage Responses and College of Life Sciences, Capital Normal University, Beijing, China

2. Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

3. Toxicology Program, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

Abstract

It is known that budding yeast PCNA sumoylation serves as a ligand to recruit a DNA helicase Srs2 through its tandem receptor motifs that prevent unwanted homologous recombination (HR) at replication forks, a process known as salvage HR. This study reveals detailed molecular mechanisms, in which constitutive PCNA-PIP interaction has been adapted to a regulatory event.

Funder

Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mbio.00315-23

Reference53 articles.

1. Regulation of alternative replication bypass pathways at stalled replication forks and its effects on genome stability: a yeast model

2. DNA Postreplication Repair Modulated by Ubiquitination and Sumoylation