Author:
Mountain A,McChesney J,Smith M C,Baumberg S
Abstract
From a partial Sau3A gene library of Bacillus subtilis chromosomal DNA in the expression plasmid pRK9, four hybrid plasmids were isolated carrying overlapping segments of the argA-argF-cpa cluster. The complementation patterns within Escherichia coli arginine auxotrophs of these hybrids and deletion derivatives provided the gene order argC-argA-argE-argB-argD-cpa-argF.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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