PCR identification of Mycobacterium bovis BCG

Author:

Talbot E A1,Williams D L1,Frothingham R1

Affiliation:

1. Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA.

Abstract

The attenuated bacillus Calmette-Guérin (BCG) vaccine strain is derived from a virulent strain of Mycobacterium bovis. BCG is difficult to differentiate from other strains of M. bovis and other members of the M. tuberculosis complex by conventional methods. Recently, a genomic region designated RD1 was found to be present in all virulent M. bovis and M. tuberculosis strains tested but deleted from all BCG strains tested. With this information, a multiplex PCR method was developed to detect the RD1 deletion. A large collection of BCG and other M. tuberculosis complex strains from diverse host and geographic origins was tested. RD1 was deleted in 23 of 23 BCG strains. RD1 was present in 129 of 129 other M. tuberculosis complex strains. This multiplex PCR method can be used as a tool for the rapid and specific identification of BCG.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference33 articles.

1. Subdivision of daughter strains of bacille Calmette Guerin (BCG) according to secreted protein patterns;Abou-Zeid C.;J. Gen. Microbiol.,1986

2. American Type Culture Collection. 1992. Catalogue of bacteria and bacteriophages 18th ed. American Type Culture Collection Rockville Md.

3. Bacillus Calmette-Guerin immunotherapy;Brosman S. A.;Urol. Clin. North Am.,1992

4. IS6110: conservation of sequence in the Mycobacterium tuberculosis complex and its utilization in DNA fingerprinting;Cave M. D.;Mol. Cell. Probes,1991

5. Usefulness of IS6110 in fingerprinting DNA of Mycobacterium bovis;Cave M. D.;Med. Microbiol. Lett.,1992

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3