Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients-Reference-Cited by-同舟云学术

Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients

Published:1997-03 Issue:3 Volume:35 Page:788-790
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Gallez-Hawkins G M¹,Tegtmeier B R¹,ter Veer A¹,Niland J C¹,Forman S J¹,Zaia J A¹

Affiliation:

1. Department of Pediatrics, City of Hope National Medical Center, Duarte, California 91010, USA.

Abstract

A plasma PCR test, using a nonradioactive PCR plate assay, was evaluated for detection of human cytomegalovirus reactivation. This assay was compared to Southern blotting and found to perform well. As a noncompetitive method of quantitation, it was similar to a competitive method for detecting the number of genome copies per milliliter of plasma in marrow transplant recipients. This is a technically simplified assay with potential for adaptation to automation.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.35.3.788-790.1997

Reference24 articles.

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